OLT Crystal Shifter devices contribute to the global fight against COVID-19
Diamond scientists were supported by OLT’s Crystal Shifter devices and software to solve a new structure of the SARS-CoV-2 main protease (MPro), and complete an XChem crystallographic fragment screen against it.
In just 72 hours, 600 optimized crystals have been harvested and analysed. Thousands of more crystals followed, on one day over 1,000 crystals have been harvested before lunch! Such high optimization and harvesting troughput was enabled by Oxford Lab Technologies’ Crystal Shifter devices and software. The harvesting results were directly fed to XChem’s analytical pipeline for further processing. Diamond’s daily data sets are made available freely to the global scientific community.
“By the 24th of March, the initial 1500-crystal experiment was complete, and the results made publicly available… This was an exceptionally large screen which yielded a remarkably rich readout, with vast opportunities for fragment growing and merging.”
Diamond Light Source, XChem Lab
We are humbled our devices and software are helping scientists at Diamond and contributing to such an important global research effort.
Read more in Nature Communications 2020.
Diamond Light Source (UK)
MAX IV Synchrotron (Sweden)
University of California, San Francisco (USA)
Academic Papers
Nature Communications 2020: “Crystallographic and electrophilic fragment screening of the SARS-CoV-2 main protease“
Acta Crystallographica 2020: “FragMAX: the fragment-screening platform at the MAX IV Laboratory“
Acta Crystallographica 2019: “The Low-Cost, Semi-Automated Shifter Microscope Stage Transforms Speed and Robustness of Manual Protein Crystal Harvesting“
Acta Crystallographica 2018: “Where is crystallography going?“
Essays in Biochemistry 2017: “The SGC beyond structural genomics: redefining the role of 3D structures by coupling genomic stratification with fragment-based discovery“
MiTeGen
Structural Genomics Consortium (SGC)
Centre for Medicines Discovery
